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</div> <div class="container-fluid clearfix layout_564394 text-center text-bold" style="font-size: 20px; color: rgb(0, 0, 0); margin-top: 30px; margin-bottom: 15px;"> <div class="column"> <div class="container clearfix layout_439253"> <div class="column"> <div class="column visual-conent-title"> <p class="content-title"> 血清细胞外囊泡含有原发性硬化性胆管炎和胆管癌的蛋白生物标志物</p> <div class="clearfix"> </div> </div> </div> </div> </div> </div> <div class="container-fluid clearfix layout_610181" style="margin-top: 0px;"> <div class="column"> <div class="container clearfix layout_148235 text-center text-bold" style="font-size: 20px; color: rgb(0, 0, 0);"> <div class="column"> <div class="hr"> </div> </div> </div> </div> </div> <div class="container-fluid clearfix layout_369638 text-center" style="margin-top: 15px; margin-bottom: 15px;"> <div class="column"> <div class="container clearfix layout_13448"> <div class="column"> <div class="col-md-12 layout_455380"> <div class="col-md-2 column"> </div> <div class="col-md-8 column"> <div class="column visual-conent-author visual-inline-block"> <p class="content-author"> <span>作者：</span>admin</p> </div> <div class="column visual-conent-adddate visual-inline-block" style="padding-left: 15px; padding-right: 15px;"> <p class="content-adddate"> <span>添加时间：</span>2019-04-15 10:07:44</p> </div> <div class="column visual-conent-source visual-inline-block"> <p class="content-source"> <span>来源：</span>永诺生物</p> </div> <div class="column visual-category-statistics visual-inline-block" style="padding-left: 15px;"> 浏览：<script src="/index.php?case=archive&act=view_js&aid=40"></script></div> </div> <div class="col-md-2 column"> </div> </div> </div> </div> </div> </div> <div class="container-fluid clearfix layout_617573" style="margin-top: 15px; margin-bottom: 15px;"> <div class="column"> <div class="container clearfix layout_903271"> <div class="column"> <div class="column visual-category-title"> <div class="content_tools_box"> <p class="content_tools"> <a href="javascript:CallPrint('print');">打印本文</a> <a href="javascript:doZoom(14)">小</a> <a href="javascript:doZoom(18)">中</a> <a href="javascript:doZoom(20)">大</a> </p> <div class="clearfix"> </div> </div> </div> </div> </div> </div> </div> <div class="container-fluid clearfix layout_43304" style="margin-top: 15px; margin-bottom: 15px;"> <div class="column"> <div class="container clearfix layout_784828"> <div class="column"> <div class="column visual-conent"> <p><strong>题目</strong>：Serum extracellular vesicles contain protein biomarkers for primary sclerosing cholangitis and cholangiocarcinoma</p><p><strong>期刊</strong>：Hepatology</p><p><strong>影响因子</strong>：11.711</p><p><strong>简介</strong>：胆管癌（CCA）是一类胆道系统癌症，预后差。原发性硬化性胆管炎（PSC）等病因是胆管癌的危险因素。肝内胆管癌（ICCA）与肝细胞癌（HCC）进行非侵入性鉴别诊断有时是困难的。 目前尚未有PSC、CCA和HCC的准确非侵入性生物标志物。为寻找新生物标志物，研究人员从CCA（n = 43）、PSC（n = 30）和HCC（n = 29）患者和健康个体（对照组，n = 32）中分离出血清细胞外囊泡（EV），并检测其蛋白质含量。通过使用纳米粒子跟踪分析（NTA），发现HCC血清EV浓度高于其他各组。圆形形态（透射电子显微镜）、大小（NTA为〜180nm直径）和标记物（免疫印迹检测CD9、CD63和CD81）表明，大多数血清EV是外泌体。</p><p>蛋白质组图谱（通过质谱法）显示出多个差异表达的蛋白。这些蛋白中的几种显示出较高的诊断价值，CCA vs对照组的ROC曲线下最大面积（AUC）为0.878，CCA II期vs对照组为0.905，PSC vs对照组为0.789，PSC非肝硬化vs对照组为0.806， CCA vs PSC为0.796，CCA Ⅱ期vs PSC为0.956，HCC vs对照组为0.904，iCCA vs HCC为0.894。通过对人CCA细胞EV的蛋白质组学分析显示，与正常人胆管细胞释放的EV相比，其致癌蛋白的丰度更高。在免疫缺陷小鼠的肝脏中原位植入人CCA细胞，导致含有类似人致癌蛋白的EV在血清中释放。</p><p style="text-align: center;"><img src="/html/upload/images/201904/15563545223814.png" width="632" height="683"/></p></div> </div> </div> </div> </div> <div class="container-fluid clearfix layout_676599" style="margin-top: 15px; margin-bottom: 15px;"> <div class="column"> <div class="container clearfix layout_158624"> <div class="column"> <div class="hr"> </div> </div> </div> </div> </div> <div class="container-fluid clearfix layout_667472"> <div class="column"> <div class="container clearfix layout_381979"> <div class="column"> <div class=" visual-conent-form"> </div> </div> </div> </div> </div> <div class="container-fluid clearfix layout_886423" style="margin-top: 15px; margin-bottom: 5px;"> <div class="column"> <div class="container clearfix layout_390441"> <div class="column"> <div class="column visual-conent-page"> <div id="page"> <strong>上一篇</strong><a href="/index.php?case=archive&act=show&aid=39">多参数血浆EV分析有助于胰腺恶性肿瘤诊断</a> <div class="blank10"> </div> <strong>下一篇</strong><a href="/index.php?case=archive&act=show&aid=41">树突状细胞衍生的外泌体在本体肝细胞癌小鼠模型中引发肿瘤消退</a> </div> </div> </div> </div> </div> </div> <div class="container-fluid clearfix layout_402892" style="margin-top: 15px; margin-bottom: 15px;"> <div class="column"> <div class="container clearfix layout_939884"> <div class="column"> <div class="hr"> </div> </div> </div> </div> </div> <div class="container-fluid clearfix layout_57479" style="margin-top: 15px; margin-bottom: 15px;"> <div class="column"> <div class="container clearfix layout_608164"> <div class="column"> <div class="column visual-conent-comment"> </div> </div> </div> </div> </div> <div class="container-fluid clearfix layout_815628" style="margin-top: 15px; margin-bottom: 15px;"> <div class="column"> <div class="container clearfix layout_771703"> <div class="column"> <div class="hr"> </div> </div> </div> </div> </div> <div class="container-fluid clearfix layout_275539" style="margin-top: 15px; margin-bottom: 15px;"> <div class="column"> <div class="container clearfix layout_875600"> <div class="column"> <div class="column visual-conent-relevant-list"> <h3> <a><a href='/index.php?case=tag&act=show&tag=%E5%A4%96%E6%B3%8C%E4%BD%93' target='_blank'>外泌体</a> <a href='/index.php?case=tag&act=show&tag=%E8%A1%80%E6%B8%85' target='_blank'>血清</a> <a href='/index.php?case=tag&act=show&tag=%E8%83%86%E7%AE%A1%E7%99%8C' target='_blank'>胆管癌</a> <small>相关内容</small></a> </h3> <span>——</span> <hr class="featurette-divider" /> <div class="container list-container"> <div class="news-list list-border list-border-w"> <div class="list-date"> <span>21</span> <p> 2019-03</p> </div> <h4> <a title=""新宠"外泌体正式进入临床检验，路还有多远？" href="/index.php?case=archive&act=show&aid=65" target="_blank">"新宠"外泌体正式进…</a> </h4> <p> 一管血检测癌症，是全球医学界共同的梦想。液体活检作为传统活检的替代技术，通过非侵入性的取样方式获得肿瘤信息，辅助癌症治疗，是精准医疗代表性的诊断技术。液体活检的主要检测物包括血液中的循环肿瘤细胞(circulating tumor cells，CTCs)、循环肿瘤DNA(circ… <a href="/index.php?case=archive&act=show&aid=65" target="_blank">[了解更多]</a> </p> </div> <div class="news-list list-border list-border-w"> <div class="list-date"> <span>11</span> <p> 2019-01</p> </div> <h4> <a title="2018外泌体重量级研究一览，看这篇就够了" href="/index.php?case=archive&act=show&aid=64" target="_blank">2018外泌体重量级研究一览，看这篇就够</a> </h4> <p> 刚过去的2018年，也是外泌体研究热度持续升温的一年，在多个领域均涌现了重大的研究进展。本篇结合Nature、Cell、Cell Metab、Gastroenterology、Science、Nat Rev Mol Cell Biol、Chem Rev发表的重量级文章对2018外…… <a href="/index.php?case=archive&act=show&aid=64" target="_blank">[了解更多]</a> </p> </div> <div class="news-list list-border list-border-w"> <div class="list-date"> <span>20</span> <p> 2019-04</p> </div> <h4> <a title="2019年恩赛尔正式推出外泌体标准化服务" href="/index.php?case=archive&act=show&aid=50" target="_blank">2019年恩赛尔正式推出外泌体标准化服务</a> </h4> <p> 2019年4月厦门恩赛尔生物科技有限公司正式完成平台升级与系统完善，面向工业客户提供标准化的外泌体分离提纯及检测服务。… <a href="/index.php?case=archive&act=show&aid=50" target="_blank">[了解更多]</a> </p> </div> <div class="news-list list-border list-border-w"> <div class="list-date"> <span>01</span> <p> 2019-02</p> </div> <h4> <a title="2018年ncell参与外泌体行业共识制定" href="/index.php?case=archive&act=show&aid=49" target="_blank">2018年ncell参与外泌体行业共识制</a> </h4> <p> 2018年12月公司首席科学家叶国栋博士作为执笔人参与国内首个外泌体行业共识的制定与发布。… <a href="/index.php?case=archive&act=show&aid=49" target="_blank">[了解更多]</a> </p> </div> <div class="news-list list-border list-border-w"> <div class="list-date"> <span>11</span> <p> 2019-04</p> </div> <h4> <a title="Exosome：揭秘干细胞与免疫细胞如何“对话”" href="/index.php?case=archive&act=show&aid=48" target="_blank">Exosome：揭秘干细胞与免疫细胞如何</a> </h4> <p> 在此网络研讨会中，Pluchino博士讲解了外泌体与神经干细胞和神经元前体细胞发生信号传导的情况，以及它们在神经炎性疾病中的作用。此外，他还谈及外泌体靶向治疗的未来。… <a href="/index.php?case=archive&act=show&aid=48" target="_blank">[了解更多]</a> </p> </div> </div> <div class="clearfix"> </div> </div> </div> </div> </div> </div> </div> <div class="view-html"> <div class="container-fluid clearfix layout_971667" style="padding-top: 60px; padding-bottom: 60px; background-color: rgb(51, 51, 51); color: rgb(255, 255, 255);"> <div class="column"> <div class="container clearfix layout_976890 text-bold" style="font-size: 20px; padding-top: 20px; padding-bottom: 20px;"> <div class="column"> 厦门恩赛尔生物科技有限公司位于国家级企业孵化基地海沧生物医药产业园，参照GMP标准建设有完善的研发生产实验室并通过厦门市食品药品质量检查研究院依据国家标准GB50457-2008的洁净级别检测，团队汇聚大帮技术精湛的博士、硕士人员及外籍科学家，并由多名“千人计划”专家作为技术顾问，同时兼具遗传咨询师、系统架构师、注册审核员等各类人才，具备专业的科学研究和服务咨询能力。 企业定位为工业客户提供标准化的外泌体分离提纯及检测服务，并且在该项技术领域拥有创新突破和权威认可，作为首席科学家兼企业法人代表，叶国栋博士在肿瘤及血液检测技术领域有十数年的技术积累，参与并执笔国内首个《外泌体研究、转化和临床应用专家共识》，未来将继续促进该项标准化技术的落地，为外泌体行业的蓬勃发展贡献一份力量。</div> </div> <div class="container clearfix 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